Equipment/Instrumentation

TIRF

 

TIRF

Modes available

  • Contact, AC (tapping) and dual AC, 1-D forces, lateral force, nanolithography, electrostatic force, Kelvin probe, conductive AFM, magnetic force, piezoelectric force, thermal AFM

Applications: Mechanics of cells, tissues, and polymers. Tagging and identification of cells, proteins, and molecules. Controlled dosing of samples via micropipette. Controlled environment (fluid, temperature) experiments.

Scan range

  • 90μm × 90μm × 15 μm closed loop

Z noise

  • <0.06nm 0.1Hz-1kHz BW

Cantilever noise

  • <0.02nm Adev, 0.1Hz-1kHz BW

Accessories

  • Micromanipulator, nano-injector
  • Temperature-controlled chamber
  • Environmental fluid cell

Bio prep-space

  • CO2 incubator, Bio-safety cabinet

Optics

  • 641, 532, 488 nm lasers
  • Cascade II EMCCD camera with dual view filter
  • Nikon inverted optical microscope
  • 10×, 20×, 40×, 100× objectives

 

TIRF